Trichoderma pseadokoningiRifi|BNCC336348 |BNCC

Trichoderma pseadokoningiRifi

Price： $ 115
number：BNCC336348
Form：
Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the plate hyphae is white, yellow spores are produced, spread to the edge of the plate, and the back of the medium is yellow.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

Add to cart

View Cart

Essential Information Certificate Related Products

Trichoderma pseadokoningiRifi

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;3-5 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the plate hyphae is white, yellow spores are produced, spread to the edge of the plate, and the back of the medium is yellow.
Sharing mode	Public welfare sharing

Trichoderma pseadokoningiRifi

Description

1,BNCC No.:336348

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1, Comprehensive PDA:20% potato juice 1L, glucose 20g, KH2PO43 g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural, 121 ℃,15min.

2.Atmosphere: aerobic

3,.Temperature: 25-28 ℃,5-7 days

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

Download certificate