BeNa Culture Collection

Botryotinia squamosa-BNCC
Botryotinia squamosa-BNCC
Botryotinia squamosa-BNCC
  • BNCC
  • Botryotinia squamosa-BNCC
  • Botryotinia squamosa-BNCC
  • Botryotinia squamosa-BNCC

Botryotinia squamosa

  • Price: $ 100
  • number:BNCC336329
  • Form:
    Small filamentous fungi, the colonies are obvious on the comprehensive PDA medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, and the back of the medium is golden yellow.
Standard strain Quantitative strain DNA extraction
Package:
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Botryotinia squamosa
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions 28 ℃;5-7 days; Aerobic;
Storage conditions 2-8 ℃
morphology Small filamentous fungi, the colonies are obvious on the comprehensive PDA medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, and the back of the medium is golden yellow.
application medicinal
Sharing mode Public welfare sharing

1.Description 

1. Name: Poriacocos(Fr.)Wolf

2. BNCC No.:336329

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions 

1. Medium composition: potato extract 1.0L, glucose 20.0g, agar 15.0g,pH natural.

[note] potato extract: take 200g of peeled potato, cut into small pieces, add 1.0L of water and boil for 30min, filter out potato pieces, and supplement the filtrate to 1.0L.

2. Atmosphere:aerobic

3.Temperature: 28-32 ℃

4.Notes: 

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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