Penicillium chrysogenum Thom|BNCC336234 |BNCC

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Penicillium chrysogenum Thom

Culture medium	CDA: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water and 6.2±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Spread growth, producing a large number of spores, producing yellow spores, irregular edges, opaque, flat shape, rough surface, surface folds, dry texture, purity: pure, gray on the front, gray on the back
Sharing mode	Public welfare sharing

Penicillium chrysogenum Thom

Storage conditions : 2~8 ℃

No. : 336234

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :25-28 ℃, aerobic, Cha's agar, 5-7 days. Cha's agar: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water, pH 6.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 2 plates;

(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability, in 5-7 days strain layer is obvious and colony is typical
colony morphology: (above)	filamentous fungi have obvious colonies on Cha's agar medium, hyphae are flourishing and growing, the edge mycelium is white, the texture is fluffy, the conidia structure is large, and blue-green spores are produced.
conclusion	good viability, no abnormal colony morphology, qualified

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