Paecilomyces variotii|BNCC336231 |BNCC

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Paecilomyces variotii

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;3-5 days; Aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread growth, large number of spores, irregular edges, opaque, gray-white front, gray-white back, flat shape, rough surface, wrinkled surface, dry texture, pure
Model strain	no
application	Anti-mildew test bacteria, GBT 1741 "detection method for mold resistance of paint film" experimental mold, "environmental test of electronic and electrical products part 2: experimental method test j and guidelines: mildew growth" (GB/T 2423.16-2008) requires strains to be used
Sharing mode	Public welfare sharing

Paecilomyces variotii Bainier

Storage conditions : 2~8 ℃

No. : 336231

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Growth conditions :25-28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA:20% potato liquid 1.0L, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, vitamin B1 8mg, agar 20.0g,pH natural. 121 ℃,15min. 20% potato liquid configuration: take 200g of peeled potato, boil for 30min, filter to take filtrate, and make up to 1.0l.

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability, in 6 days strain layer is obvious
colony morphology	small filamentous fungi have obvious colonies on the integrated PDA medium. the hyphae are white cotton wool-like at the beginning, dense and vigorous, and later produce yellow-green spores. the back of the medium is light yellowish brown.
conclusion	good viability, no abnormal colony morphology, qualified

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