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Aspergillus nidulans
Storage conditions : 2~8 ℃
No.: 336164
Product format: freeze dried, 200ul
Validity period: 6 years
Biosafety level: 1 , handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.
Growth conditions:28 ℃, aerobic, chashel agar, 5-7 days, chashel agar: 3g sodium nitrate, 1g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 0.5g potassium chloride, 0.01g ferrous sulfate, 30g sucrose, 15g agar, 1.0L distilled water, pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Recovery steps: (1)Prepare 1-2 of above mentioned plates; (2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps; (3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; (4)Put the plates under the above culture conditions for cultivation for 5-7 days
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows: