abnormal hansenes yeast|BNCC336003 |BNCC

Essential Information Certificate Related Products

abnormal hansenes yeast

Culture medium	YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;24-48h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: round Edge: neat Transparency: Opacity Color: milky white Protuberance: Middle bulge Surface: bright and smooth Texture: moist and viscous
application	Increase the ester content of the wine and make it fragrant.
Sharing mode	Public welfare sharing

Hansenula anomala

storage conditions : 2~8 ℃

number: 336003

product format: freeze dried, 200ul

Validity: 6 years

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:28 ℃, aerobic, Shah glucose agar, 48h. Shah's glucose agar: 40g glucose, 10g peptone (casein trypsin digest, gastric enzyme digest of animal tissue), 15g agar, 1.0L distilled water, pH 5.6 ±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viability:	good viability. in 20 hours liquid medium become turbid, the strain layer is obvious;colony is typical on marked plate
Colony morphology: (as shown below)	Size: 1-2mm; Shape: Round; Edge: Neat; Transparency: Opaque Color: milky white; middle raised surface: bright and smooth texture: moist and viscous
Conclusion:	good viability, no abnormal colony morphology, qualified

Download certificate