BeNa Culture Collection

Neisseria sicca (von Lingelsheim) Bergey et al.-BNCC
Neisseria sicca (von Lingelsheim) Bergey et al.-BNCC
  • BNCC
  • Neisseria sicca (von Lingelsheim) Bergey et al.-BNCC
  • Neisseria sicca (von Lingelsheim) Bergey et al.-BNCC

Neisseria sicca (von Lingelsheim) Bergey et al.

  • Price: $ 343
  • number:BNCC329833
  • Form:
    Round, neat edges, opaque, light color, light color, convex in the middle, smooth and bright surface, moist texture, easy to pick up, no pigment, G-(red), cocci, purity: pure
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Neisseria sicca (von Lingelsheim) Bergey et al.
Culture medium Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;18-24h; Atmosphere 5% CO2 + 95% air;
Storage conditions 2-8 ℃
morphology Round, neat edges, opaque, light color, light color, convex in the middle, smooth and bright surface, moist texture, easy to pick up, no pigment, G-(red), cocci, purity: pure
application Media testing.
Sharing mode Public welfare sharing

Neisseria sicca

Storage conditions: 2~8 ℃

No. 329833

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃,5% CO2, Columbia blood plate (ready-to-use type),18-24h.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates; 

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; 

(4) Put the plates under the above culture conditions for cultivation for 18-24 hours.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                                                  

Item test results
viability good viability,in 18-24h strain layer become obvious,  colony is typical
colony morphology: (above)

Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque

Color: off-white uplift: middle raised surface: bright and smooth texture: moist and viscous

Conclusion good viability, no abnormal colony morphology, qualified
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