BeNa Culture Collection

Corynebacterium striatum (Chester) Eberson-BNCC
  • BNCC
  • Corynebacterium striatum (Chester) Eberson-BNCC

Corynebacterium striatum (Chester) Eberson

  • Price: $ 372
  • number:BNCC327370
  • Form:
    Size: 2-4mm Shape: round Edge: not neat Transparency: Opacity Color: light yellow Protuberance: Middle bulge Surface: Smooth Texture: easy to stir up
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Corynebacterium striatum (Chester) Eberson
Culture medium Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37°C;24-48h; Aerobic;
Storage conditions 2-8 ℃
morphology Size: 2-4mm Shape: round Edge: not neat Transparency: Opacity Color: light yellow Protuberance: Middle bulge Surface: Smooth Texture: easy to stir up
application Quality control strain
Sharing mode Public welfare sharing

Corynebacterium striatum

Storage conditions: 2~8 ℃

No. 327370

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, brain heart extract agar, 24-48h. Brain heart extract agar: brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 
(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; 
(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                             

item test results
viability good viability, in 24-48h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology (above) size: 2-4mm shape: round edge: irregular transparency: opaque color: light yellow uplift: middle convex surface: smooth texture: easy to stir
Conclusion good viability, no abnormal colony morphology, qualified
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