BeNa Culture Collection

Streptococcus agalactiae-BNCC
Streptococcus agalactiae-BNCC
  • BNCC
  • Streptococcus agalactiae-BNCC
  • Streptococcus agalactiae-BNCC

Streptococcus agalactiae

  • Price: $ 143
  • number:BNCC316406
  • Form:
    Size: 2-4mm Shape: round Edge: neat Transparency: Opacity Color: white Protuberance: Flat Surface: bright and smooth Texture: moist and viscous, neat edges, front white, light color, convex in the middle, smooth and bright surface, moist texture, easy to pick up, no pigment, G + (blue-purple), cocci, purity: pure
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Streptococcus agalactiae
Culture medium Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
morphology Size: 2-4mm Shape: round Edge: neat Transparency: Opacity Color: white Protuberance: Flat Surface: bright and smooth Texture: moist and viscous, neat edges, front white, light color, convex in the middle, smooth and bright surface, moist texture, easy to pick up, no pigment, G + (blue-purple), cocci, purity: pure
application Media testing
Control strain for Streptococcus Group B
Sharing mode Public welfare sharing

Streptococcus agalactiae

Storage conditions: 2~8 ℃

No.: 316406

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level: 2, handle in safety cabinet 

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, brain heart extract agar, 18-24h. Brain heart extract agar: 4.0g of bovine brain extract, 4.0g of bovine heart extract, 5.0g of peptone, 16.0g of casein peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, 20.0g of agar (not included in liquid medium),pH7.4 ± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 
(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; 
(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

item test result
viability good viability,in 18-24h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.

colony morphology:

(above)

Size: 2-4mm Shape: Round Edge: Neat Transparency: Opaque

color: white uplift: flat surface: bright and smooth texture: moist and viscous

Conclusion good viability, no abnormal colony morphology, qualified
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