Cryptococcus neoformans var. grubii|BNCC290220 |BNCC

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Cryptococcus neoformans var. grubii

Culture medium	YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃,24-48h, aerobic
Storage conditions	2-8 ℃
morphology	The colony diameter is 2-4mm, round, with neat edges, opaque, milky white on the front, milky white on the back, convex in the middle, bright surface, smooth surface, moist texture, easy to stir up, G + (blue-purple), cocci, purity: pure
Separation substrate	Hodgkin's disease patients, fresh york
application	Biomedical research and development materials Research on Emerging Infectious Diseases Negative control in differential gene expression studies Produce laccase
Sharing mode	Public welfare sharing

Cryptococcus neoformans var. grubii

Storage conditions: 2~8 ℃

No.: 290220

Product format: freeze dried,200ul

Validity period: freezed-dried tube 6 years

Biosafety level:1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28-30 ℃, aerobic, YM medium. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2,121 ℃,15min.

Recovery steps:
(1)Prepare 1-2 YM plates;
(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4)Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 40h,YM plate marking colony is obvious
colony morphology	size: small color: milky white shape: round edge: neat edge wet and dry: moist and smooth: slightly rough transparency: opaque uplift: uplift
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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