Clostridium sporogenes (Metchnikoff) Bergey et al.|BNCC288716 |BNCC

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Clostridium sporogenes (Metchnikoff) Bergey et al.

Culture medium	Liquid thioglycolate medium (FT): 15.0g of caseptone (trypsin hydrolysis), 5.0g of yeast extract, 5.0g of glucose, 0.5g of sodium thioglycolate, 0.5g of L-cystine, 2.5g of sodium chloride, 0.001g of azure, 0.75g of agar, 1.0L of distilled water and 7.1±0.2 of pH value. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1 φ18mm test tube with 10ml of liquid medium, or two fresh plates ( deaeration in anaerobic environment for 24 hours before handling); (2) sterilization of the ampoule, open it in the cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃,24-48h, anaerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Off-white Protuberance: Flat Surface: Grey Dark Texture: Wet
Separation substrate	Gas gangrene
application	Media testing Quality control strain Sterility testing Pharmaceutical and Personal Care United States Pharmacopoeia USP-32-NF 27, 2009 bacteria for microbiological testing The Pharmacopoeia specifies bacteria for microbiological testing, sterility test
Sharing mode	Public welfare sharing

Clostridium sporogenes

Storage conditions: 2~8 ℃

No.: 288716

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, FT medium, 48-72h. FT medium: caseptone (trypsin hydrolysis) 15.0g, yeast extract 5.0g, glucose 5.0g, sodium thioethanolate 0.5g,L-cystine 0.5g, sodium chloride 2.5g, azure 0.001g, agar 0.75g, distilled water 1.0L,pH 7.1 ± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a φ18mm test tube of 10ml liquid medium;

(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Put the test tube under the above culture conditions for cultivation. The culture solution is obviously turbid or mass growth at the solution bottom occur, this indicates the bacterial grows well.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 48-72h liquid medium is turbid, strain grow slowly on the plate
colony morphology: (above)	Clostridium sporogenes is gram-positive bacilli. FT liquid culture is turbid and plate strains are weak. liquid culture is recommended.
Conclusion	good viability, no abnormal colony morphology, qualified

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