Aspergillus brasiliensis Varga et al.|BNCC286403 |BNCC

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Aspergillus brasiliensis Varga et al.

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for 18-24 hours.
Growth conditions	28 ℃,5-7 days, aerobic
Storage conditions	2-8 ℃
morphology	Spread growth, black spore production, front black, dark, purity: pure
Separation substrate	Blueberry, North Carolina
application	U.S. Pharmacopoeia USP-32-NF 27, 2009 Microbiological Testing Bacteria Pharmacopoeia Specify Microbiological Testing Bacteria, Antimicrobial Efficacy Test, Counting Test of Non-sterile Products, Sterility Test, CTFA (American Cosmetic Flavor Association) Recommended Cosmetic Microbiological Challenge Bacteria
Sharing mode	Public welfare sharing

Aspergillus brasiliensis

Storage conditions: 2~8 ℃

No. 286403

Product format :freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, 5-7 days, comprehensive PDA agar medium: potato boiling solution 1.0L, glucose 20.0g,KH₂PO₄ 3.0g,MgSO₄·7H₂O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability，in 3 days floc grows at the top of the culture solution, and the plate bacteria layer is obvious.In 5 days strain layer become obvious, colony is typical on the streaked plate
Colony morphology (pictured above)	filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae, dense/low flat, The hyphae spread vigorously and produced a large number of black spores.
Conclusion	good viability, no abnormal colony morphology, qualified

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