Streptococcus equi subsp. zooepidemicus Farrow and Collins|BNCC281388 |BNCC

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Streptococcus equi subsp. zooepidemicus Farrow and Collins

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare 1-2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for 18-24 hours.
Growth conditions	37 ℃,18-24h, aerobic
Storage conditions	2-8 ℃
morphology	Size: 2-4mm shape: irregular edge: neat transparency: translucent Color: off-white bulge: middle bulge surface: bright and smooth texture: moist and sticky
Separation substrate	Nitrosoguanidine-induced mutant of clinical isolate HA-100
application	Produces hyaluronic acid Production of high molecular weight hyaluronic acid
Sharing mode	Public welfare sharing

Description

1. Name: HA-116 Streptococcus equi subsp. zooepidemicus Farrow and Collins

2. BNCC No.: 281388

3. Biosafety level:3

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, TSA/TSB (tryptone soybean medium): tryptone 17.0g, soy peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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