Actinomadura sp.|BNCC276654 |BNCC

Actinomadura sp.

Price： $ 372
number：BNCC276654
Form：
Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: yellow uplift: middle convex surface: rough texture: dry

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Actinomadura sp.

Culture medium	Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions.
Growth conditions	30 ℃,2-3 days, aerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: yellow uplift: middle convex surface: rough texture: dry
Separation substrate	Soil
application	Produces A 40926 complex
Sharing mode	Public welfare sharing

Description

1. Name:Actinomadura sp.

2. BNCC No.: 276654

3.Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, ISP-1 medium (Yeast Culture Medium): tryptone 5.0g, yeast extract 3.0g, agar 20.0g, distilled water 1.0L,pH 7.0-7.2. Sterilization at 121 ℃ for 15min.

2. Atmosphere: aerobic

3, Temperature: 25-28 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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