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Bordetella pertussis
Storage conditions: 2~8 ℃
No. 272535
Product format: freeze dried, 200ul
Validity : 6 years
Biosafety level: 2 , handle in safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :37 ℃, aerobic, Colombian blood plate (ready-to-use type),2-3 days.
Recovery steps: (1) Prepare 1-2 of above mentioned plates; (2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; (4) Put the plates under the above culture conditions for cultivation for 2-3 days. Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque
color: white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir