BeNa Culture Collection

Streptomyces threomyceticus-BNCC
Streptomyces threomyceticus-BNCC
  • BNCC
  • Streptomyces threomyceticus-BNCC
  • Streptomyces threomyceticus-BNCC

Streptomyces threomyceticus

  • Price: $ 572
  • number:BNCC261096
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Streptomyces threomyceticus
Culture medium Yeast starch agar (A): yeast extract (BD  Difco)2.0g, soluble starch 10.0g, agar 15.0g, distilled water 1.0L,pH 7.3,121 ℃,15mim sterilization.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;3-4 days; Aerobic
Storage conditions 2-8 ℃
Sharing mode Public welfare sharing

 Streptomyces threomyceticus

Storage conditions: 2~8 ℃

No. 261096

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, yeast starch agar (A),3-4 days. Yeast starch agar (A): yeast extract (BD Difco)2.0g, soluble starch 10.0g, agar 15.0g, distilled water 1.0L,pH 7.3,121 ℃,15mim sterilization.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability: good viability,in 3 days strain layer become obvious,  colony is typical
colony morphology: (above)

Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque

Color: white bulge: middle bulge surface: rough texture: dry and difficult to stir

Conclusion: good viability, no abnormal colony morphology, qualified

 

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