Corynebacterium durum Riegel et al.|BNCC254239 |BNCC

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Corynebacterium durum Riegel et al.

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for 48-72 hours.
Growth conditions	37 ℃,2-3 days, anaerobic
Storage conditions	2-8 ℃
morphology	Size: 0.5-1mm shape: round edge: irregular transparency: opaque Color: off-white bulge: middle bulge surface: rough gray texture: moist and easy to stir up
Separation substrate	Subgingival plaque
Sharing mode	Public welfare sharing

Corynebacterium durum

Storage conditions : 2~8 ℃

No. : 254239

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, strictly anaerobic, Colombian blood plate (ready-to-use type),48-72h.

Recovery steps:
(1) Prepare 2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water(keep in an anaerobic environment for 24 hours) into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 5-7 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 48h strain layer is obvious; colony is typical on the scribed plate,
colony morphology: (above)	size: 0.5-1mm shape: round edge: irregular transparency: opaque color: off-white bulge: middle bulge surface: rough gray texture: wet and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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