Streptomyces peucetius Grein et al.|BNCC250285 |BNCC

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Streptomyces peucetius Grein et al.

Culture medium	Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1- 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions.
Growth conditions	28 ℃,3-5 days, aerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: brown bulge: middle bulge surface: rough texture: dry
Separation substrate	Mutant derived by disruption of the dnrII gene in S. Peucetius ATCC 29050
Sharing mode	Public welfare sharing

Streptomyces peucetius Grein et al.

Storage conditions: 2~8 ℃

No. 250285

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, ISP-2 medium, 3-5 days. ISP-2 medium: yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, agar 15.0g, distilled water 1.0 L,pH7.3,121 ℃,15min.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 3 days strain layer become obvious, colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Irregular Edge: Irregular Transparency: Opaque color: brown uplift: middle raised surface: rough texture: dry
Conclusion:	good viability, no abnormal colony morphology, qualified

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