Haemophilus influenzae (Lehmann and Neumann) Winslow et al.|BNCC248915 |BNCC

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Haemophilus influenzae (Lehmann and Neumann) Winslow et al.

Culture medium	Columbia agar chocolate plate (CA-C): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast leaching powder 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 80-90 ℃, then add 5% sterile defibrated sheep blood, shake well and pour the plate to cool for later use.
Subculture procedure	(1) Prepare 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for18-24 hours.
Growth conditions	37 ℃,18-24h,5% CO2 + 95% air
Storage conditions	2-8 ℃
morphology	Size: 2-4mm shape: round edge: neat transparency: opaque Color: off-white uplift: middle convex surface: bright and smooth texture: moist, neat edges, off-white on the front, dark, smooth and bright surface, moist texture, easy to stir up, no pigment, G-(red), bacilli, purity: pure
Separation substrate	Throat of young adult with respiratory infection
application	Respiratory research
Sharing mode	Public welfare sharing

Haemophilus influenzae

Storage conditions: 2~8 ℃

No. 248915

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃,5% CO₂, chocolate plate (ready to use),18-24h.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 18-24h strain layer become obvious, colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability， no abnormal colony morphology, qualified

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