Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz|BNCC244941 |BNCC

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Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz

Culture medium	Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃,18-24h, aerobic
Storage conditions	2-8 ℃
morphology	Size: 0.5-1mm shape: round edge: neat transparency: opaque Color: off-white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir up
application	Assay of sulfamethoxazole Assay of trimethoprim Evaluation of Mueller-Hinton agar Quality control strain Susceptibility disc testing Susceptibility testing Quality control strain for Difco products
Sharing mode	Public welfare sharing

Enterococcus faecalis

Storage conditions : 2~8 ℃

No. 244941

Product format: freeze dried, 200ul

Validity : 6 years

biosafety level : 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, brain heart infusion medium BHI,18-24h. Brain heart extract medium BHI: brain extract powder 5.0g, heart extract powder 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 18-24h liquid medium become turbid, strain layer is obvious; colony is typical on the streaked plate
colony morphology: (above)	size: 0.5-1mm shape: round edge: neat transparency: opaque Color: off-white uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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