Streptococcus thermophilus|BNCC232113 |BNCC

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Streptococcus thermophilus

Culture medium	MRS medium (MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a φ18mm test tube containing 25mL of liquid medium. Silica gel plug is used for the liquid test tube; sit it under anaerobic environment for 24 hours. (2) sterilize the ampoule, use a small grinding wheel to make a circle at 1/2 of the tube body under strict aseptic conditions, and then break it; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate. (4) transfer the solution to the liquid test tube, plug with silica gel; (5) Put the liquid test tube under the above culture conditions for cultivation; the culture solution is obviously turbid or mass growth at the solution bottom occur, this indicates the bacterial grows well.
Growth conditions	37 ℃,3-5 days, anaerobic
Storage conditions	2-8 ℃
Separation substrate	Lesai brand probiotic capsules
application	Probiotics research.
Sharing mode	Public welfare sharing

Streptococcusthermophilus

No. :232113

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The freeze dried culture shall be used up once and shall not be retained. Handling instructions of suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, MRS medium, 18-24h. MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, distilled water 1.0L. pH 5.7±0.2. 121 ℃, 15min sterilization

Recovery steps:
(1)Prepare a φ18mm sterile test tube of 25ml liquid medium; Silica gel plug is used for the liquid test tube;
(2) Sterilize the ampoule, use a small grinding wheel to make a circle at 1/2 of the tube body under strict aseptic conditions,, and then break it
(3) Sraw 0.5ml of liquid growth medium into a freeze dried ampoule, make it full dissolved;
(4) Transfer all the solution to the liquid test tube, plug the tube with silica gel.
(5) Place the liquid test tube in incubator under the above growth conditions for cultivation, The culture solution is obviously turbid or precipitated, and this indicates the bacterial grows well.

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