Bacillus|BNCC231922 |BNCC

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Bacillus

Culture medium	Base medium (LB): yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃,18-24h, aerobic
Storage conditions	2-8 ℃
morphology	Size: Medium Color: Yellow Shape: Round Edge: Neat Edge Wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: low convex
Separation substrate	Sun salt field soil
application	Anaerobic fermentation to produce lactic acid. Azo dyes can be decolorized under high salt concentration conditions. Flocculation properties of extracellular polymers.
Sharing mode	Public welfare sharing

Bacillussp.

Storage conditions: 2~8 ℃

No.: 231922

Product format: freeze dried,200ul

Validity period: freeze-dried tube for 24 months

Biosafety level: 1, handle in ultra-clean table or biosafety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture:37°C, aerobic, LB broth medium. LB broth medium: peptone 10.0g, yeast powder 5.0g,NaCl10.0g, distilled water 1.0L,pH7.0.

Recovery steps:
(1)Prepare a flask of LB liquid media.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min);

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 20h,LB bacterial fluid is turbid, LB plate colony is obvious
colony morphology:	size: medium color: yellow shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: low convex
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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