Pseudomonas chlororaphis subsp.aurantiaca|BNCC231888 |BNCC

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Pseudomonas chlororaphis subsp.aurantiaca

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃,18-24h, aerobic
Storage conditions	2-8 ℃
morphology	Size: general color: yellowish shape: round edge: neat edge Wet and Dry: Wet Smooth: Smooth Transparency: Opaque Protuberance: Protuberance
Separation substrate	Plant rhizosphere soil
application	It has a growth-promoting effect and has an antagonistic effect on fungal diseases. The antibiotics phenazin-1-carboxylic acid (PCA) and the auxin indole -3-acetic acid (IAA) are produced. It can be used for microbial teaching research and also for the preparation of microbial fertilizers.
Sharing mode	Public welfare sharing

Pseudomonas g chlororaphis subsp.

No.: 231888

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: Freeze-dried tube for 24 months

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28-30 ℃, aerobic, nutrient gravy medium (NB). Nutritional gravy medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, distilled water 1.0L, pH7.0. 121 ℃,15min.

Recovery steps:
(1)Prepare a flask of NB liquid media.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min).

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 20h NB bacterial fluid is turbid; The bacterial liquid is marked NA plate with obvious colony
colony morphology:	size: general color: yellowish shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque uplift: bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified.

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