Oligotrophomonas spp.|BNCC231875 |BNCC

Oligotrophomonas spp.

Price： $ 143
number：BNCC231875
Form：
Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: yellow uplift: middle convex surface: smooth and bright texture: moist and easy to stir up

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Oligotrophomonas spp.

Culture medium	Base medium (LB): yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃,18-24h, aerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: yellow uplift: middle convex surface: smooth and bright texture: moist and easy to stir up
Separation substrate	Seaside beach
application	It can simultaneously reduce hexavalent chromium and adsorb divalent zinc.
Sharing mode	Public welfare sharing

Stenotrophomonassp.

BNCC No.:231875

Biosafety level: 4

Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. LB medium: yeast extract 5.0g peptone 10.0g NaCl 10.0g agar 15.0g distilled water 1.0 L pH 7.0.

2. Atmosphere: aerobic

3. Temperature: 30 ℃

Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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