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Cortinarius rufo-olivaceus
BNCC No.:230932
Storage conditions: 2~8 ℃
Product format: agar slant in 14mm test tube
Validity period: 30 days
Biosafety level: 1, handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:25-28 ℃, aerobic, PDA. PDA: potato extract 6g, glucose 20g, agar 20g,pH natural, 121 ℃,15min.
Recovery steps:
(1)Prepare 1-2 pieces of PDA plates;
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;
(4) Lay flat the small pieces to the center of the agar plate;
(5)Put the agar plates under the above culture conditions for cultivation 5-7days
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
small filamentous fungi are obviously visible on integrated PDA agar medium,
colonies are pink-white, dry, vigorous, spread on the surface of the culture medium and do not produce spores.