Streptomyces roseoflavus|BNCC228869 |BNCC

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Streptomyces roseoflavus

Culture medium	Gauche Synthesis No. 1 Agar (GM): 20g of soluble starch, 0.5g of sodium chloride, 0.01g of ferrous sulfate, 1.0g of potassium nitrate, 0.5g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 15g of agar, 1.0L of distilled water and 7.3±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for 2-3 days.
Growth conditions	30 ℃,2-3 days, aerobic
Storage conditions	2-8 ℃
morphology	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: white bulge: middle bulge surface: rough fold texture: firm hard dry
application	Produced metabolites have antibacterial activity
Sharing mode	Public welfare sharing

1.Description

1. Name：Streptomyces roseoflavus

2. BNCC No.：228869

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Gao's synthetic agar No. 1: soluble starch 20.0g,KNO3 1.0g,K2HPO4 0.5g,MgSO4 ·7 H2O 0.5g,NaCl 0.5g,FeSO4 0.01g, agar 15.0g, distilled water 1.0 L,pH 7.2-7.4. 121 ℃,15min.

2. Atmosphere：aerobic

3. Temperature： 28 ℃

4.Notes：

1.Normal culturing time：24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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