Grifola frondosa|BNCC228383 |BNCC

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Grifola frondosa

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of PDA plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃,5-7 days, aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with distinct colonies on PDA medium, white, raised, spreading to the edge of the plate and growing without spore production.
Sharing mode	Public welfare sharing

Grifola frondosa

BNCC No.： 228383

storage conditions: 2~8 ℃

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 dyas

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, PDA,5-7 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (not included in liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability,in 5-7 days, strain layer is obvious
colony morphology:	small filamentous fungi, with obvious, white and convex colonies on PDA medium, spread and grow to the edge of the plate without spores.
conclusion:	good viability, no abnormal colony morphology, qualified

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