Klebsiella sp.|BNCC196052 |BNCC

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Klebsiella sp.

Culture medium	Base medium (LB): yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a fllak containing LB liquid medium; (2) Open the ampoule in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid medium, mix evenly; cultivate in a shaker at 28 ℃ (140r /min) for 18-24hours.
Growth conditions	30 ℃,18-24h, aerobic
Storage conditions	2-8 ℃
morphology	Size: medium color: orange shape: round edge: neat edge Wet and dry: wet and smooth: smooth transparency: opaque uplift: middle bulge
Separation substrate	Sisanoni Mature Fruit
Sharing mode	Public welfare sharing

Klebsiella sp.

BNCC No.：196052

Storage conditions: 2~8 ℃

Product format： freeze dried, 200ul

Validity period: freeze-dried tube for 24 months

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, LB broth medium. LB broth medium: peptone 10.0g, yeast powder 5.0g,NaCl 10.0g, distilled water 1.0L,pH7.0.

Recovery steps:

(1)Prepare a flask of LB liquid media

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 28℃ for 18-24 hours (140r/min);

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 20h LB bacterial fluid is turbid, LB plate colony is obvious
colony morphology:	size: medium color: orange yellow shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque bulge: middle bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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