Fusarium proliferatum|BNCC195647 |BNCC

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Fusarium proliferatum

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of PDA plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃,3-5 days, aerobic
Growth characteristics	Colony morphology: The average growth rate of the bacterium on PDA medium was 7mm/d, and the whole plate was covered in 12 days. The reverse side of the medium substrate is dark yellow, the colony is white, fluffy and convex, cotton wool-like, dense. Microscopic morphology of hyphae (400 ×): The hyphae were clearly separated and more, with a diameter of 3-5μm. The conidiophores were relatively straight, with sausage-shaped apices and no obvious spores were found. Bacterial morphology in the fermentation broth: the bacterial liquid is slightly turbid, the hyphae in the fermentation broth are white clouds, and no bubbles are found on the liquid surface. The production of mycotoxins inhibits the growth of certain plants.
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on the integrated PDA medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, The back of the medium is orange-yellow.
Separation substrate	Oxytropis vulgaris plant tissue. Collection: Inner Mongolia Alxa League Zuoqi Ha
application	Research. Fermentation produces the anti-tumor active drug marisin (swainsonine). The polar segment of ethyl acetate, the fermentation product, has good antibacterial activity.
Sharing mode	Public welfare sharing

1.Description

1. Name：Fusarium proliferatum

2. BNCC No.：195647

3. Biosafety level： 4

2.Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Medium composition: potato extract 1.0L, glucose 20.0g, agar 15.0g,pH natural. [Note] Potato extract: 200g of peeled potato is taken, cut into small pieces, add 1.0L of water to boil for 30min, filter out potato pieces, and supplement the filtrate to 1.0L.

2. Atmosphere：aerobic

3. Temperature： 25 ℃

4.Notes

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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