Verticillium lecanii|BNCC195401 |BNCC

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Verticillium lecanii

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃,5-7 days, aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies, white hyphae, dense and low flat, almost no spread on comprehensive PDA medium
application	Insect pathogenic bacteria; Biological control
Sharing mode	Public welfare sharing

1.Description

1. Name：Verticillium lecanii

2. BNCC No.：195401

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Comprehensive PDA: potato boiled juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

2. Atmosphere：aerobic

3. Temperature： 25-28 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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