Clostridium sp|BNCC195293 |BNCC

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Clostridium sp

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃,24-48h, anaerobic
Storage conditions	2-8 ℃
morphology	Size: 1-4mm Shape: Irregular Edge: Irregular Transparency: Opacity Color: milky white uplift: flat surface: bright and smooth texture: moist and viscous
application	Caproic acid is produced and liquor is fragrant.
Sharing mode	Public welfare sharing

1.Description

1. Name：Clostridium kluyveri

2. BNCC No.：195293

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Thioglycolate medium: caseptone 15.0g, yeast leaching powder 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, azure 0.001g, sodium chloride 2.5g,pH7.1±0.2. 121 ℃,15min.

2. Atmosphere：anaerobic

3. Temperature：37 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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