Bacillus mucilaginosus|BNCC195271 |BNCC

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Bacillus mucilaginosus

Culture medium	Nitrogen-free medium: nitrogen-free medium: mannitol (or glucose) 10.0g, potassium dihydrogen phosphate 0.2g, magnesium sulfate heptahydrate 0.2g, sodium chloride 0.2g, calcium sulfate dihydrate 0.2g, calcium carbonate 5g, agar 15g (not included in liquid medium), distilled water 1.0L,pH7.0-7.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃,24-48h, aerobic
Storage conditions	2-8 ℃
morphology	Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Transparent Color: white bulge: middle bulge surface: smooth and bright texture: moist and viscous
Separation substrate	Soil
application	Bacterial fertilizer, potassium bacteria
Sharing mode	Public welfare sharing

Bacillus mucilaginosus

BNCC No.：195271

Biosafety level：4

2.Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1.Silicate bacteria culture medium: sucrose 5.0g, disodium hydrogen phosphate 2.0g, magnesium sulfate 0.5g, ferric chloride 0.005g, calcium carbonate 0.1g, bauxite 0.5g,pH7.1±0.2. 121 ℃,15min.

2. Atmosphere：aerobic

3. Temperature：25 ℃

4.Notes

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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