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BNCC > Microbial strain > Staphylococcus sciuri
Staphylococcus sciuri-BNCC
  • BNCC
  • Staphylococcus sciuri-BNCC

Staphylococcus sciuri

  • Price： $ 143
  • number：BNCC195019
  • Form：
    Size: 0.5-1mm shape: round edge: neat transparency: opaque Color: milky white uplift: middle convex surface: bright and smooth texture: moist
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Certificate Related Products
Staphylococcus sciuri
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic
Storage conditions 2-8 ℃
morphology Size: 0.5-1mm shape: round edge: neat transparency: opaque Color: milky white uplift: middle convex surface: bright and smooth texture: moist
Separation substrate Fermented beef
application Study on Fermented Meat Products
Sharing mode Public welfare sharing

Staphylococcus sciuri

 

1.Description

BNCC No.：195019 

Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions 

1, Mannitol agar (Mannitol Agar) medium composition: yeast powder 5.0g, peptone 3.0g, mannitol 25.0g, agar 15.0g, distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

2. Atmosphere：aerobic

3. Temperature：30 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization
 

 

 

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