Aspergillus carbonarius|BNCC193598 |BNCC

Aspergillus carbonarius

Price： $ 143
number：BNCC193598
Form：
Filamentous fungi, the colonies are obvious on the Cha's agar medium, the hyphae spread vigorously and grow to cover the entire plate, the initial hyphae are gray-white and dense, and the later hyphae turn black and produce black spores.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Aspergillus carbonarius

Culture medium	CDA: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water and 6.2±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Filamentous fungi, the colonies are obvious on the Cha's agar medium, the hyphae spread vigorously and grow to cover the entire plate, the initial hyphae are gray-white and dense, and the later hyphae turn black and produce black spores.
application	Morphological Identification of Tannase Producing, Pectinase, Cellulase and GB 4789.16-2016
Sharing mode	Public welfare sharing

Aspergillus carbonarius

BNCC No.：193598

Biosafety level：4

Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. Synthesis PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling liquid:200g potato, peel, cut into pieces, boil in distilled water 30min, take the filtrate to 1000mL, for later use. 121 ℃,15min.

2. Atmosphere： aerobic

3. Temperature：25-28℃

Notes：
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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