BeNa Culture Collection
Culture medium | MRS medium (MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min. |
Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
Growth conditions | 37 ℃;48-72h; Micro aerobic (3%-5% O2,10% CO2) |
Storage conditions | 2-8 ℃ |
morphology | Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: milky white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir up |
Separation substrate | Soak pig ears |
application | Fermented pickles, fruit and vegetable products, feed, etc. |
Sharing mode | Public welfare sharing |
Pediococcus pentosaceus
No.:192624
Product format: freeze dried, 200ul
Storage conditions: 2~8 ℃
Validity period: 6 years
Biosafety level: 1 , handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.
Growth conditions:37 ℃, micro aerobic, MRS medium. MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. 121 ℃,15min sterilization
Recovery steps:
(1)Prepare a φ18mm test tube of 10ml liquid medium and 2 plates;
(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4)Put the test tube under the above culture conditions for cultivation. The culture solution is obviously turbid or mass growth at the solution bottom occur, this indicates the bacterial grows well.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
item | test result |
viability | good viability, in 48-72h liquid medium become turbid, obvious strain layer occurs on the plate; colony is typical on marked plate |
colony morphology: (above) | size: 1-2mm shape: round edge: neat transparency: opaque color: milky white uplift: middle raised surface: bright and smooth texture: moist and easy to stir |
Conclusion | good viability, no abnormal colony morphology, qualified |