Salmonella saintpaul|BNCC192087 |BNCC

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Salmonella saintpaul

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic
Storage conditions	2-8 ℃
morphology	Size: general color: yellowish shape: round edge: neat edge Dry and wet: wet and smooth: smooth transparency: opaque uplift: bulge
Separation substrate	Imported Frozen Turkey Two-section Wing
application	Research, quality control
Sharing mode	Public welfare sharing

Salmonella saintpaul

Storage conditions: 2~8 ℃

No.: 192087

Product format: freeze dried, 200ul

Validity period: Freeze-dried tube for 6 years

Biosafety level: handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Activation/passage:37°C, aerobic, nutrient agar medium (NA). Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

①Prepare 1 bottle of NA liquid culture medium (100ml triangular bottle is divided into 50ml culture medium) or 2 NA plates;

②Open the safety tube in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③Suck 0.5mL of liquid culture medium and put it into a freeze-drying tube. after fully dissolving, all the bacteria suspension will be beaten back into the liquid culture medium, mixed evenly, and cultured in a 37 ℃ shaker. Or directly suck the bacterial suspension (200 & mu; L/a) hit NA plate, evenly coated, the plate placed in a 37 ℃ incubator for 18-24h.

Recovery record: According to the activation requirements, the above strains were resuscitated, and the recorded results are as follows:

item	test result
viability	good viability, in 20h NB bacterial liquid is turbid, colony is typical on marked plate
colony morphology	size: general color: yellowish shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque uplift: bulge
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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