Monascus purpureus|BNCC190826 |BNCC

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Monascus purpureus

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on PDA medium, dense and vigorous hyphae, later producing orange spores, spreading to the edge of the plate Growth, the back of the medium is reddish brown, producing red pigment., Dark color, dark color, purity: pure
Separation substrate	Sufu
application	Research on red pigment production
Sharing mode	Public welfare sharing

Description

1. Name: Monascus anka

2. BNCC No.: 190826

3. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions

1. Wort agar medium: 130g malt paste powder, 15g agar, 0.1g chloramphenicol, 1L distilled water, pH6.0±0.2,121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 28 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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