Dark-colored Charcoal cluster bacteria|BNCC190529 |BNCC

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Dark-colored Charcoal cluster bacteria

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi have obvious colonies on the comprehensive PDA medium, the hyphae will turn yellow in the early white and late stage, the hyphae will be dense and vigorous, spreading to the edge of the plate, and the back of the medium will be light black.
application	Used in the production of edible fungi.
Sharing mode	Public welfare sharing

Annulohypoxylon stygium

No.: 190529

Storage conditions: 2~8 ℃

Product format: agar slant in 14mm test tube

Validity period: growing culture in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, integrated PDA agar, 6 days. Comprehensive PDA agar: potato boil 1.0L, glucose 20.0g,KH₂PO₄ 3.0g,MgSO₄·7H₂O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 6 days plate colony is obvious
colony morphology:	small filamentous fungi with obvious colonies, light yellow and dense hyphae on integrated PDA culture medium, the edge hyphae are white and spread to concentric circles on the edge of the plate. the back of the culture medium has a black center.
conclusion:	good viability, no abnormal colony morphology, qualified

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