Penicillium griseofulvum|BNCC187812 |BNCC

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Penicillium griseofulvum

Culture medium	Malt juice agar (MA): malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Growth characteristics	The texture of the colony is flocculent and thick; the color of the colony is light green; the surface of the colony has radial grooves, and the center is slightly raised; the edge is flat; there is a small amount of colorless exudate; the colony forms a large number of spores, which produce significant stalks in the marginal area. The conidia are subspherical, green, with smooth surface; the sporophyte is single curved, with smooth walls and bundles; the stem base is short; the broom branches are loose and scattered; the secondary branches are scattered.
Storage conditions	2-8 ℃
morphology	The texture of the colony is flocculent and thick; the color of the colony is light green; the surface of the colony has radial grooves, and the center is slightly raised; the edge is flat; there is a small amount of colorless exudate; the colony forms a large number of spores, which produce significant stalks in the marginal area.
application	Research, griseofulvin production.
Sharing mode	Public welfare sharing

1. Description

1. Name: Penicillium griseofulvum

2. BNCC No.: 187812

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

2. Atmosphere: aerobic

3. Temperature: 25-28 ℃,5-7 days

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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