Streptomyces mobaraensis|BNCC186555 |BNCC

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Streptomyces mobaraensis

Culture medium	YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;2-3 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: white uplift: flat surface: rough texture: dry
application	Research; Production
Sharing mode	Public welfare sharing

Streptomyces mobaraensis

Storage conditions: 2~8 ℃

No. 186555

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30°C, aerobic, YM medium, 2-3 days. YM medium: yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L, pH 6.2±0.2. Sterilize at 121°C for 15min.

Recovery steps:

① Prepare 1-2 pieces of Blood agar plates;

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③ Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well in 200ml/plate;

④ Put the agar plates under the above culture conditions for cultivation，2-3days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 3days strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 2-4mm ; Shape: round ; Edge: irregular ; Transparency: opaque color: white ; Protuberance: flat ; Surface: rough ; Texture: dry
Conclusion:	good viability, no abnormal colony morphology, qualified

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