Bifidobacterium longum|BNCC186496 |BNCC

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Bifidobacterium longum

Culture medium	Bifid enrichment medium (BBL): peptone 15.0g, glucose 20.0g, yeast extract 2.0g, soluble starch 0.5g, sodium chloride 5.0g,L-cysteine 0.5g, tomato powder 5.0g, liver powder 2.0g, Tween 80 1mL, agar 20.0g (excluding liquid), distilled water 1.0L,pH value 7.0±0.1. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;3-5 days; Anaerobic;
Storage conditions	2-8 ℃
Separation substrate	Infant intestinal tract
application	Production of restriction enzymes BinSI,BinSII; for probiotic health food production.
Sharing mode	Public welfare sharing

Bifidobacterium longum

Description

1. BNCC No.: 186496

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1, BBL liquid medium: peptone 15g, glucose 20g, yeast powder 2g, soluble starch 0.5g, sodium chloride 5g, L-cysteine 0.5g, tomato powder 5g, liver powder 2g, Tween 80 1g,pH 6.8±0.2. 115 ℃,20min.

2. Atmosphere: anaerobic

3. Temperature: 37 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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