BeNa Culture Collection
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1. Description
1. Name: Bifidobacterium longum
2. BNCC No.: 186483
3. Biosafety level: 4
2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C.
3. Growth Conditions:
1. MRS medium + 0.05% cysteine:(MRS medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1000mL. pH6.2±0.2.121 ℃,15min.)
2. Atmosphere: anaerobic
3. Temperature: 37 ℃
4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.
2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.
3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.
4.Waste generated from the handling process should be discarded after high-pressure sterilization.