BeNa Culture Collection
Home
Cell Products
Microbe Products
Download
Quality Control Products
News
My Account
About Us
Streptococcus equinus
No. 186309
Storage conditions: 2~8 ℃
Product format: freeze dried,200ul
Validity period: 6 years
Biosafety level: 1, handle in ultra-clean table or safety cabinet
Receving notices:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:37 ℃, aerobic, BHI: 4.0g of bovine brain extract powder, 4.0g of bovine heart extract powder, 5.0g of peptone, 16.0g of casin peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, pH7.4±0.2. 121 ℃,15min.
Recovery steps:
(1)Prepare a flask of BHI liquid media or two BHI agar plates.
(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a BHI agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
size: small color: milky white shape: round edge: neat edge
wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: uplift