Vibrio parahaemolyticus|BNCC186296 |BNCC

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Vibrio parahaemolyticus

Culture medium	3.5% sodium chloride agar medium (NA-2): beef paste 3g, peptone 10.0g,NaCl 35.0g, agar 20.0g (not included in liquid medium),pH 7.2~7.4. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic;
Growth characteristics	G-bacilli, which cannot grow on salt-free medium, propagates rapidly in 3% ~ 6% saline, with a cycle every 8~9 minutes, and cannot grow in less than 0.5% or higher than 8% saline. Motivation, oxidase positive; Lysine decarboxylase and ornithine decarboxylase are positive, arginine dihydrolase is negative, indole is positive, urease and volt-pu test are negative; salt-free peptone water, 1% salt peptone water does not grow, 6%, 8% salt peptone water grows; fermentation of glucose, mannitol, maltose, arabinose, rhamnose, sucrose does not produce gas; does not ferment lactose, salicylate. Facultative anaerobic, optimum pH7.4 ~ 7.6.
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, golden yellow on the front and golden yellow on the back, flat shape, smooth and bright surface, moist texture, easy to stir up, G-(red), bacilli, purity: pure
application	Research, quality control, "GB 4789.7-2013 Vibrio parahaemolyticus test" positive control strain, "GB 4789.28 medium and reagent quality requirements" standard strain. "GB 4789 National Food Safety Standard Food Microbiological Inspection" Standard Strain Recommendation
Sharing mode	Public welfare sharing

Vibrio parahaemolyticus

Storage conditions: 2~8 ℃

No.: 186296

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, 3.5% sodium chloride agar medium, 18-24h. 3.5% sodium chloride agar medium: beef paste 3g, peptone 10.0g,NaCl 35.0g, agar 20.0g (not included in liquid medium), distilled water 1L,pH7.2 ~ 7.4,121 ℃,15min sterilization.

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 18-24h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	size: 2-4mm shape: round edge: neat transparency: opaque color: yellow uplift: flat surface: bright and smooth texture: moist and viscous
Conclusion	good viability, no abnormal colony morphology, qualified

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