Kluyveromyces lactis|BNCC186194 |BNCC

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Kluyveromyces lactis

Culture medium	Glucose agar (SDA): 40g glucose, 10g peptone (casein trypsin digest, gastric enzyme digest of animal tissue), 15g agar (not included in liquid medium), 1.0L distilled water, pH 5.6±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;24-48h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: general color: milky white shape: round edge: neat edge dry and wet: wet and smooth: smooth and bright transparency: opaque uplift: convex
Sharing mode	Public welfare sharing

Kluyveromyces lactis

No.: 186194

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: freeze-dried tube 24 months

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice：if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Agar plates can be used directly. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28-30 ℃, aerobic, Shah glucose agar (SDA). SDA medium: glucose 40g, peptone (casein trypsin digest, gastric enzyme digest of animal tissue) 10g, agar 15g, distilled water 1.0L,pH 5.6±0.2,121 ℃, 15min sterilization.

Recovery steps:

(1) Prepare 1-2 pieces of SDA plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a SDA agar plate evenly.

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 40h SDA plate scribing colony is obvious
colony morphology:	size: general color: milky white shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque bulge: bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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