Rhodococcus horses|BNCC186169 |BNCC

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Rhodococcus horses

Culture medium	Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic;
Growth characteristics	G + cocci, contact enzyme positive, urease positive, no power, biochemical reaction inactive, do not ferment any sugars and alcohols. facultative anaerobic.
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Translucent Color: Light Yellow Protuberance: Middle Protuberance Surface: Smooth and Bright Texture: Wet and Viscous
application	Research; Analytical testing. Research, quality control, "GB 4789.30-2010 Listeria monocytogenes test" negative control strain.
Sharing mode	Public welfare sharing

Rhodococcus equi

BNCC No.:186169

Biosafety level: 3

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1, Medium composition: heart extract 5.0g, brain extract 12.5g, monthly peptone 10.0g, glucose 2.0g,NaCl 5.0g, na ₂HPO₄2.5g, agar 15.0g, distilled water 1.0L,pH7.4.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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