Shewanella putrefaciens|BNCC186122 |BNCC

Shewanella putrefaciens

Price： $ 143
number：BNCC186122
Form：
The colony diameter is 1-2mm, round, with neat edges, opaque, golden yellow on the front, golden yellow on the back, convex in the middle, smooth and bright surface, moist texture, easy to stir up, no pigment, G-(red), bacilli, purity: pure

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Shewanella putrefaciens

Culture medium	Tryptone soybean medium (TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃; Aerobic;
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, golden yellow on the front, golden yellow on the back, convex in the middle, smooth and bright surface, moist texture, easy to stir up, no pigment, G-(red), bacilli, purity: pure
application	Research, quality control
Sharing mode	Public welfare sharing

Shewanella putrefaciens

BNCC No.: 186122

Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1. Tryptone soybean agar (TSA): tryptone (casein trypsin digest) 15.0g, soybean peptone (soybean powder papain digest) 5.0g, sodium chloride 5.0g, agar 15.0g,pH7.3±0.2. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 30 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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