Phanerochaete chrysosporium|BNCC186045 |BNCC

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Phanerochaete chrysosporium

Culture medium	Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;3-5 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on PDA medium, white hyphae, dense and vigorous, low and flat bacterial layer, and light yellow on the back of the medium.
application	Decompose lignin.
Sharing mode	Public welfare sharing

Phanerochaete chrysosporium

Storage conditions: 2~8 ℃

No. 186045

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, PDA,3-5 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (not included in liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 4 days, plate colony is obvious
colony morphology:	small filamentous fungi with obvious colonies and white hyphae on PDA medium, dense and vigorous, the bacterial layer is low and flat, and the back of the culture medium is light yellow
Conclusion:	good viability, no abnormal colony morphology, qualified

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