Listeria monocytogenes|BNCC185986 |BNCC

Essential Information Certificate Related Products

Listeria monocytogenes

Culture medium	Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic;
Growth characteristics	G Corynebacterium brevis, dynamic, catalase positive, β-hemolysis on the blood plate; negative nitrate reduction test, negative urease test, no fermentation of xylose and mannitol; positive methyl red reaction, positive Volt-Pu test, fermentation of glucose, maltose, heptaside, rhamnose; trisugar iron test produces acid, no hydrogen sulfide; facultative anaerobic, the most suitable pH7.4 ~ 7.6.
Storage conditions	2-8 ℃
morphology	Round, neat edges, opaque, light color, light color, convex in the middle, smooth and bright surface, moist texture, easy to pick up, no pigment, G + (blue-purple), bacilli, purity: pure
application	Research; Analytical testing. Research, quality control, "GB 4789.30-2010 Listeria monocytogenes test" positive control strain.
Sharing mode	Public welfare sharing

Listeria monocytogenes

No.: 185986

Product format: freeze dried,200ul

Storage conditions: 2~8 ℃

Validity period: 6 years

Biosafety level: 2, handle in biosafety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, BHI: 4.0g of bovine brain extract powder, 4.0g of bovine heart extract powder, 5.0g of peptone, 16.0g of casin peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, pH7.4±0.2. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of BHI liquid media or two BHI agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a BHI agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 24h BHI bacterial fluid is turbid, BHI plate colony is obvious
colony morphology:	size: smaller color: white shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: middle bulge
Conclusion:	good viability， and colony morphology is not abnormal, completely consistent with the above figure, qualified

Download certificate