Serratia marcescens|BNCC185953 |BNCC

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Serratia marcescens

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	26 ℃;18-24h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: Small Color: Orange Red Shape: Round Edge: Neat Dry and Wet: Wet and Smooth: Smooth Transparency: Opaque Protuberance: Long
application	Filter membrane testing; Sterility testing; Antibacterial preparation testing; Antibacterial hand washing formula product testing.
Sharing mode	Public welfare sharing

Serratia marcescens

Storage conditions: 2~8 ℃

No. 185953

Product format: freeze dried, 200ul

Validity period: Freeze-dried tube for 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Activation/passage:26 ℃, aerobic, nutritious gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a test tube containing liquid culture medium or 2 plates;

(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 26℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 26℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 20h liquid medium become turbid, obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology	Size: Small Color: Orange Red Shape: Round Edge: Neat Wet and dry: moist smooth: smooth transparency: opaque uplift: uplift
Conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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